An acidic solution
of monosulfonic azo dye, Acid Orange 12, reacts with
basic groups on protein to form an insoluble complex. The
protein=s dye binding capacity (DBC)
is genetically controlled and is essentially constant for
any given protein system. The estimate of protein content
is based on colorimetric measurement of unbound dye. The
dye serves as a primary standard.
Applicable to raw or
UDY-Pol preserved milk from all dairy breeds as well as
to modified milk such as homogenized, pasteurized, skim,
UDY Model MKP
Protein Analyzer including an UDY Dairy Tester
(Colorimeter) along with Standard Reagent and Reference
- Drain Bottle
and Tubing - Attach the end of the Drain Tube
(feed tubing up through the bottom of the unit)
to the Cuvet Tip. It may be necessary to moisten
the tip of the Cuvet with water to make the
attachment easier. Wipe off any fingerprints on
the Cuvet with a soft tissue or a soft cloth.
- After the
tubing is attached, seat the Cuvet Stop making
sure that the scribe mark on the top of the Cuvet
faces the front.
- Connect the
Power Supply to the unit and plug the Power
Supply transformer into the appropriate outlet.
Make sure the unit switch is in the OFF
position before plugging in the unt.
- Turn on and
warm up the unit for 30 minutes before operating.
- Follow the
operating instructions below.
- Fill the Cuvet
with demineralized water. Start the flow-through
process by gently squeezing the Drain Bottle.
Place your finger over the vent hole of the Drain
Bottle Cap and release.
Colorimeter switch to CAL position.
- Adjust ZERO
Potentiometer to read .000 on meter.
- Fill the Cuvet
with A66" Standard
Reference Dye Solution.
- Adjust GAIN
Potentiometer to read .660 on the meter.
- Place the
Colorimeter switch to %Protein position.
- Place SET
switch on appropriate channel No.1, 2,3,or 4 for
raw or homogenized milk. (See Factory Set Up
- Filter *sample
(25-30 drops or until stable) into Cuvet and read
%Protein (See Sample Prep).
Sample Prep. -
Raw/Homogenized Milk, etc.
- Warm milk
sample to 37C and mix.
- Weigh out 2.06
g (2.0 ml) of milk into a tared 60 ml
polyethylene Sample Bottle.
- Dispense40.37 g
(40 ml) Reagent Dye Solution into the Sample
Bottle. Cap the Sample Bottle and shake at least
15 - 20 seconds.
- Adjust the
temperature of the solution to 25C.
- Add the Filter
Cap Assembly to the Sample Bottle. Filter 25-30
drops into the cuvet.